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pMpGWB205
(Plasmid #68596)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 68596 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pMpGWB200
  • Backbone manufacturer
    Kohchi Lab., Kyoto University
  • Backbone size (bp) 9021
  • Modifications to backbone
    CaMV 35S promoter and Citrine coding sequence are both cloned upstream of the Gateway cassette.
  • Vector type
    Plant Expression
  • Promoter Cauliflower mosaic virus 35S promoter
  • Selectable markers
    Gentamicin
  • Tag / Fusion Protein
    • Citrine (N terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Spectinomycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DB3.1
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gateway Cloning

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMpGWB205 was a gift from Takayuki Kohchi (Addgene plasmid # 68596)
  • For your References section:

    Development of Gateway Binary Vector Series with Four Different Selection Markers for the Liverwort Marchantia polymorpha. Ishizaki K, Nishihama R, Ueda M, Inoue K, Ishida S, Nishimura Y, Shikanai T, Kohchi T. PLoS One. 2015 Sep 25;10(9):e0138876. doi: 10.1371/journal.pone.0138876. eCollection 2015. PONE-D-15-25288 [pii] PubMed 26406247