Cas9/pTREX-n Citations (9)
Originally described in: CRISPR/Cas9-Induced Disruption of Paraflagellar Rod Protein 1 and 2 Genes in Trypanosoma cruzi Reveals Their Role in Flagellar Attachment.Lander N, Li ZH, Niyogi S, Docampo R MBio. 2015 Jul 21;6(4). pii: e01012-15. doi: 10.1128/mBio.01012-15. PubMed Journal
Articles Citing Cas9/pTREX-n
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| Endogenous C-terminal Tagging by CRISPR/Cas9 in Trypanosoma cruzi. Lander N, Chiurillo MA, Vercesi AE, Docampo R. Bio Protoc. 2017 May 20;7(10). doi: 10.21769/BioProtoc.2299. PubMed |
| Improvements in the CRISPR/Cas9 system for high efficiency gene disruption in Trypanosoma cruzi. Romagnoli BAA, Picchi GFA, Hiraiwa PM, Borges BS, Alves LR, Goldenberg S. Acta Trop. 2018 Feb;178:190-195. doi: 10.1016/j.actatropica.2017.11.013. Epub 2017 Nov 22. PubMed |
| Genome Editing by CRISPR/Cas9 in Trypanosoma cruzi. Lander N, Chiurillo MA, Docampo R. Methods Mol Biol. 2019;1955:61-76. doi: 10.1007/978-1-4939-9148-8_5. PubMed |
| A CRISPR/Cas9-riboswitch-Based Method for Downregulation of Gene Expression in Trypanosoma cruzi. Lander N, Cruz-Bustos T, Docampo R. Front Cell Infect Microbiol. 2020 Feb 27;10:68. doi: 10.3389/fcimb.2020.00068. eCollection 2020. PubMed |
| A novel mechanosensitive channel controls osmoregulation, differentiation, and infectivity in Trypanosoma cruzi. Dave N, Cetiner U, Arroyo D, Fonbuena J, Tiwari M, Barrera P, Lander N, Anishkin A, Sukharev S, Jimenez V. Elife. 2021 Jul 2;10. pii: 67449. doi: 10.7554/eLife.67449. PubMed |
| Ablation of the P21 Gene of Trypanosoma cruzi Provides Evidence of P21 as a Mediator in the Control of Epimastigote and Intracellular Amastigote Replication. Teixeira TL, Chiurillo MA, Lander N, Rodrigues CC, Onofre TS, Ferreira ER, Yonamine CM, Santos JG, Mortara RA, da Silva CV, da Silveira JF. Front Cell Infect Microbiol. 2022 Feb 18;12:799668. doi: 10.3389/fcimb.2022.799668. eCollection 2022. PubMed |
| Efficient CRISPR-Cas9-mediated genome editing for characterization of essential genes in Trypanosoma cruzi. Picchi-Constante GFA, Hiraiwa PM, Marek M, Rogerio VZ, Guerra-Slompo EP, Romier C, Zanchin NIT. STAR Protoc. 2022 Apr 21;3(2):101324. doi: 10.1016/j.xpro.2022.101324. eCollection 2022 Jun 17. PubMed |
| Endogenous Sterol Synthesis Is Dispensable for Trypanosoma cruzi Epimastigote Growth but Not Stress Tolerance. Dumoulin PC, Vollrath J, Won MM, Wang JX, Burleigh BA. Front Microbiol. 2022 Jun 17;13:937910. doi: 10.3389/fmicb.2022.937910. eCollection 2022. PubMed |
| Proximity-Dependent Biotinylation and Identification of Flagellar Proteins in Trypanosoma cruzi. Won MM, Baublis A, Burleigh BA. mSphere. 2023 Jun 22;8(3):e0008823. doi: 10.1128/msphere.00088-23. Epub 2023 Apr 5. PubMed |
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