pQCXIP-GFP1-10
(Plasmid #68715)

• Purpose: Split GFP assay
• Depositing Lab: Yutaka Hata
• Publication: Kodaka et al Exp Cell Res. 2015 Aug 15;336(2):171-81. doi: 10.1016/j.yexcr.2015.06.015. Epub 2015 Jun 24.

Backbone

• Vector backbone: pQCXIP
• Backbone manufacturer: Clontech
• Modifications to backbone: GFP1-10 was amplified by PCR. The PCR product was ligated into BamHI/EcoRI site of pQCXIP-POU5 (created by insertion of POU5 into NotI/BamHI sites of pQCXIP). pQCXIP-POU5-GFP1-10 was digested with NotI/BamHI, filled in, and religated to remove POU5 resulting in pQCXIP-GFP1-10.
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Top10
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: GFP1-10
• Promoter: CMV

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (unknown if destroyed)
• 3′ cloning site: EcoRI (unknown if destroyed)
• 5′ sequencing primer: LNCX
• 3′ sequencing primer: IRES-R

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 13 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

GFP1-10 covers the first to the 10th beta strands of GFP.