pET-Galectin-8 FL
(Plasmid #68805)

• Purpose: Express Galectin-8 FL protein in Bacterial cells
• Depositing Lab: Hyun Kyu Song
• Publication: Kim et al Nat Commun. 2013;4:1613. doi: 10.1038/ncomms2606.

Backbone

• Vector backbone: pET vector
• Backbone size w/o insert (bp): 5400
• Total vector size (bp): 6457
• Modifications to backbone: 6 His tag - Thrombin & TEV cleavage site - insert
• Vector type: Bacterial Expression

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Growth instructions: Use BL21(DE3) or Rosetta(DE3) strain for protein production
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Galectin-8
• Alt name: LGALS8
• Alt name: Gal-8
• Alt name: Po66-CBP
• Species: H. sapiens (human)
• Insert Size (bp): 957
• Mutation: No mutation
• GenBank ID: CAA62904.1
• Entrez Gene: LGALS8 (a.k.a. Gal-8, PCTA-1, PCTA1, Po66-CBP)
• Promoter: T7
• Tag / Fusion Protein:
  • N-terminal 6-histidine-tag (N terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Bgl2 (not destroyed)
• 3′ cloning site: Kpn1 (not destroyed)
• 5′ sequencing primer: T7 universial primer
• 3′ sequencing primer: T7 terminator universial primer

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Addgene's QC results find M56V and R184S mutations, which are naturally existing variants and not of concern.