Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

pmCherryC2-Gephyrin P1
(Plasmid #68820)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 68820 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP C2
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4700
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Gephyrin
  • Alt name
    GPHN
  • Species
    R. norvegicus (rat)
  • Insert Size (bp)
    2200
  • Mutation
    silent mutations on internal EcoRI site. Gephyrin D566N (see depositor comment below)
  • GenBank ID
    NM022865.3
  • Entrez Gene
    Gphn (a.k.a. Geph)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site KpnI (not destroyed)
  • 5′ sequencing primer EGFP-C
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

EGFP was removed using NheI and XhoI. mCherry was amplified and inserted using NheI and XhoI sites. Gephyrin D566N mutation is a polymorphism and is not a concern for plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pmCherryC2-Gephyrin P1 was a gift from Shiva Tyagarajan (Addgene plasmid # 68820 ; http://n2t.net/addgene:68820 ; RRID:Addgene_68820)
  • For your References section:

    Regulation of GABAergic synapse formation and plasticity by GSK3beta-dependent phosphorylation of gephyrin. Tyagarajan SK, Ghosh H, Yevenes GE, Nikonenko I, Ebeling C, Schwerdel C, Sidler C, Zeilhofer HU, Gerrits B, Muller D, Fritschy JM. Proc Natl Acad Sci U S A. 2011 Jan 4;108(1):379-84. doi: 10.1073/pnas.1011824108. Epub 2010 Dec 20. 10.1073/pnas.1011824108 PubMed 21173228