Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected] Learn more

pAnc80L65
(Plasmid #68837)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 68837 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    synthetic
  • Backbone size w/o insert (bp) 5238
  • Total vector size (bp) 7446
  • Vector type
    Mammalian Expression, AAV, Synthetic Biology

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl2
  • Growth instructions
    Stbl4 is also appropriate for growth.
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Ancestral AAV Capsid Anc80L65
  • Alt name
    ANC80L65
  • Species
    Synthetic
  • Insert Size (bp)
    2208
  • Promoter Rep2

Cloning Information

Resource Information

Depositor Comments

An improved version of this plasmid from the Vandenberghe Lab is available. Please see pAnc80L65AAP (Addgene plasmid# 92307).

This plasmid, pAnc80L65 (#68837), requires cotransfection with a plasmid expressing AAP. For efficient viral vector production without cotransfecting AAP, use pAnc80L65AAP (Addgene plasmid# 92307)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pAnc80L65 was a gift from Luk Vandenberghe (Addgene plasmid # 68837 ; http://n2t.net/addgene:68837 ; RRID:Addgene_68837)
  • For your References section:

    In Silico Reconstruction of the Viral Evolutionary Lineage Yields a Potent Gene Therapy Vector. Zinn E, Pacouret S, Khaychuk V, Turunen HT, Carvalho LS, Andres-Mateos E, Shah S, Shelke R, Maurer AC, Plovie E, Xiao R, Vandenberghe LH. Cell Rep. 2015 Jul 28. pii: S2211-1247(15)00759-7. doi: 10.1016/j.celrep.2015.07.019. 10.1016/j.celrep.2015.07.019 PubMed 26235624