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PurposeExpresses SMASh tag fused to YFP at N terminus in yeast
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 68854 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepAG426GPD-ccdB
- Backbone size w/o insert (bp) 5741
- Total vector size (bp) 7310
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Vector typeYeast Expression
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Selectable markersURA3
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)XL10 Gold
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameyeastSMASh-YFP
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SpeciesHCV, Aequorea victoria
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Insert Size (bp)1569
- Promoter GPD
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Tag
/ Fusion Protein
- SMASh tag (N terminal on insert)
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer TCTAGAACTAGTGGATC
- 3′ sequencing primer GCGTGAATGTAAGCGTGAC (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pAG426GPD-ccdB-yeastSMASh-YFP was a gift from Michael Lin (Addgene plasmid # 68854 ; http://n2t.net/addgene:68854 ; RRID:Addgene_68854) -
For your References section:
Tunable and reversible drug control of protein production via a self-excising degron. Chung HK, Jacobs CL, Huo Y, Yang J, Krumm SA, Plemper RK, Tsien RY, Lin MZ. Nat Chem Biol. 2015 Jul 27. doi: 10.1038/nchembio.1869. 10.1038/nchembio.1869 PubMed 26214256
Map uploaded by the depositor.
