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pBrain-GFP-TACC3KDP(L566,567A)-shTACC3
(Plasmid #69117)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 69117 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pBrain
  • Backbone size w/o insert (bp) 5438
  • Total vector size (bp) 7952
  • Vector type
    Mammalian Expression, RNAi
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 50 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    Transforming Acidic Coiled Coil protein 3
  • Alt name
    TACC3
  • Alt name
    ERIC1
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    2514
  • Mutation
    LL (566-567aa) mutated to AA. Silent mutations in amino acids 30-33 to confer siRNA resistance.
  • GenBank ID
    NM_006342.2
  • Entrez Gene
    TACC3 (a.k.a. ERIC-1, ERIC1, Tacc4, maskin)
  • Promoter CMV
  • Tag / Fusion Protein
    • EGFP (N terminal on insert)

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site MluI (not destroyed)
  • 5′ sequencing primer -
  • 3′ sequencing primer -
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    shTACC3
  • Species
    H. sapiens (human)

Cloning Information for Gene/Insert 2

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    Human TACC3 Clone: IMAGE ID 6148176 (Clone BC106071).

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pBrain-GFP-TACC3KDP(L566,567A)-shTACC3 was a gift from Stephen Royle (Addgene plasmid # 69117 ; http://n2t.net/addgene:69117 ; RRID:Addgene_69117)
  • For your References section:

    TACC3-ch-TOG track the growing tips of microtubules independently of clathrin and Aurora-A phosphorylation. Gutierrez-Caballero C, Burgess SG, Bayliss R, Royle SJ. Biol Open. 2015 Jan 16;4(2):170-9. doi: 10.1242/bio.201410843. 10.1242/bio.201410843 PubMed 25596274