|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||69340||Plasmid sent as bacteria in agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepUC19 (modified)
- Backbone size w/o insert (bp) 2600
Vector typeWorm Expression
Growth in Bacteria
Growth Strain(s)ccdB Survival
Gene/Insert nameceBFP SEC 3xFlag
SpeciesC. elegans (nematode), Synthetic
Insert Size (bp)6628
/ Fusion Proteins
- ceBFP (mTagBFP)
- Cloning method Gibson Cloning
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
The plasmid was made based on pDD268 from Dickinson et al Genetics. 2015 Jun 3. pii: genetics.115.178335.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pUA97 was a gift from Oliver Hobert (Addgene plasmid # 69340)