Fgf4enhLV (FpG12)
(Plasmid #69444)

• Purpose: positive control plasmid for lentiviral reporter expression in ESCs
• Depositing Lab: Lisa Dailey
• Publication: Murtha et al Nat Methods. 2014 May;11(5):559-65. doi: 10.1038/nmeth.2885. Epub 2014 Mar 23.

Backbone

• Vector backbone: FUW
• Backbone manufacturer: Lois and Baltimore (ADDGENE # 14882)
• Backbone size w/o insert (bp): 9206
• Total vector size (bp): 11906
• Modifications to backbone: inserted FGF4 enhancer and minimal FGF4 promoter upstream of GFP coding sequences. Inserted human Ubiquitin promoter upstream of Hygromycin resistance gene. cloning details described in Murtha et al Nat Meth 2014
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Hygromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): Stbl2
• Copy number: Unknown

Gene/Insert 1

• Gene/Insert name: minimal FGF4 promoter
• Species: M. musculus (mouse)
• Insert Size (bp): 199

Gene/Insert 2

• Gene/Insert name: EGFP
• Insert Size (bp): 720

Gene/Insert 3

• Gene/Insert name: Ubiquitin promoter
• Species: H. sapiens (human)
• Insert Size (bp): 1192

Gene/Insert 4

• Gene/Insert name: Hygromycin resistance
• Insert Size (bp): 1038

Gene/Insert 5

• Gene/Insert name: FGF4 enhancer
• Species: M. musculus (mouse)
• Insert Size (bp): 249
• Entrez Gene: Fgf4 (a.k.a. Fgf-4, Fgf7a, Fgfk, HBGF-4, Hst1, Hstf-1, KS3, hst, hst-1, kFGF)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Please note that several discrepancies were found between Addgene's quality control and the depositor's sequence. The depositor noted that these discrepancies do NOT affect plasmid function.