Apple-53BP1trunc
(Plasmid #69531)

• Purpose: Fluorescent reporter for DNA damage
• Depositing Lab: Ralph Weissleder
• Publication: Yang et al Sci Rep. 2015 May 18;5:10129. doi: 10.1038/srep10129.

Backbone

• Vector backbone: pLVX
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 8003
• Total vector size (bp): 10184
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: 53BP1
• Alt name: TP53BP1
• Species: H. sapiens (human)
• Insert Size (bp): 1473
• Entrez Gene: TP53BP1 (a.k.a. 53BP1, TDRD30, p202, p53BP1)
• Promoter: CMV
• Tag / Fusion Protein:
  • Apple fluorescent protein (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BsrGI (not destroyed)
• 3′ cloning site: XbaI (destroyed during cloning)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: custom

Resource Information

• A portion of this plasmid was derived from a plasmid made by: 53BP1-YFP obtained from Prof. Galit Lahav at Harvard Medical School Department of Systems Biology
• Articles Citing this Plasmid:
  • 9 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Note: This construct contains amino acids 1220-1709 of 53BP1 followed by the short peptide VNDLDNSTG-STOP. This additional peptide does not affect reporter activity, and the plasmid functions as described in the associated publication.