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GG-EBNA-OCT4
(Plasmid #69537)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 69537 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    GGdest
  • Total vector size (bp) 10941
  • Vector type
    CRISPR
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    5 concatenated gRNA transcriptional cassettes targeting OCT4
  • gRNA/shRNA sequence
    human OCT4 promoter

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    EBNA-ori fragment used from Addgene Plasmid #27078.
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    GG-EBNA-OCT4 was a gift from Timo Otonkoski (Addgene plasmid # 69537 ; http://n2t.net/addgene:69537 ; RRID:Addgene_69537)
  • For your References section:

    Conditionally Stabilized dCas9 Activator for Controlling Gene Expression in Human Cell Reprogramming and Differentiation. Balboa D, Weltner J, Eurola S, Trokovic R, Wartiovaara K, Otonkoski T. Stem Cell Reports. 2015 Sep 8;5(3):448-59. doi: 10.1016/j.stemcr.2015.08.001. 10.1016/j.stemcr.2015.08.001 PubMed 26352799