pEGFP-N1_hTRIM32
(Plasmid #69541)

• Purpose: Expresses human TRIM32 fused to c-terminal EGFP
• Depositing Lab: Martin Dorf
• Publication: Fu et al PLoS Pathog. 2015 Jun 9;11(6):e1004960. doi: 10.1371/journal.ppat.1004960. eCollection 2015 Jun.

Backbone

• Vector backbone: pEGFP-N1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4692
• Total vector size (bp): 6659
• Modifications to backbone: Inserted between XhoI and BamHI sites: GCCACC [Kozak seq] - [Trim32 CDS, 1959 bp] - CG [to preserve frame].
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: tripartite motif containing 32
• Alt name: TRIM32
• Alt name: HT2A; BBS11; TATIP; LGMD2H
• Species: H. sapiens (human)
• Insert Size (bp): 1967
• GenBank ID: NM_012210.3 http://www.ncbi.nlm.nih.gov/gene/22954
• Entrez Gene: TRIM32 (a.k.a. BBS11, HT2A, LGMD2H, LGMDR8, TATIP)
• Promoter: CMV
• Tag / Fusion Protein:
  • EGFP (C terminal on backbone)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Xhoi (not destroyed)
• 3′ cloning site: BamHI (not destroyed)
• 5′ sequencing primer: CGTGTACGGTGGGAGGTCT (pEGFP-N1 For) or CMV forward
• 3′ sequencing primer: TCCAGCTCGACCAGGATG (pEGFP-N1 Rev)

Resource Information

• A portion of this plasmid was derived from a plasmid made by: Template was MGC clone of I.M.A.G.E. consortium from the PlasmID Repository, http://plasmid.med.harvard.edu/
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry