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CYP2C19
(Plasmid #69606)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 69606 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    CYP2C19
  • Species
    H. sapiens (human)
  • Mutation
    deletion of AA1-8 and mutation I331V
  • GenBank ID
    NM_000769.2
  • Entrez Gene
    CYP2C19 (a.k.a. CPCJ, CYP2C, CYPIIC17, CYPIIC19, P450C2C, P450IIC19)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NdeI (not destroyed)
  • 3′ cloning site HindIII (not destroyed)
  • 5′ sequencing primer pCW-SEQ-fwd (5'ACATCGTATAACGTTACTGG-3')
  • 3′ sequencing primer pCW-SEQ-rev (5'-CTTTCGTCTTCAAGCAGATCTG-3')
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

Modification of CYP2C19 for bacterial expression as per Barnes et al. PNAS 88:5597, 1991 [PMID: 1829523]: First 8 amino acids of CYP2C cDNAs replaced with those of bovine 17α hydroxylase (MALLLAVF) to optimize bacterial expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CYP2C19 was a gift from Joyce Goldstein (Addgene plasmid # 69606 ; http://n2t.net/addgene:69606 ; RRID:Addgene_69606)