PurposeUntargetted Bacterial Mutagenesis
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||69668||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerDavid Liu Lab
- Backbone size w/o insert (bp) 2992
Vector typeBacterial Expression
Growth in Bacteria
Growth Strain(s)NEB Turbo
Copy numberHigh Copy
Gene/Insert namednaQ926 dam seqA emrR ugi APOBEC1
- Promoter E. coli PBAD
- Cloning method Ligation Independent Cloning
- 5′ sequencing primer pBAD-F
- 3′ sequencing primer CAT-F (Common Sequencing Primers)
Terms and Licenses
The authors strongly recommend that all MP-carrying strains be maintained in rich media supplemented by 25 mM glucose. The expression of the MP-borne mutators is regulated by the E. coli arabinose pBAD promoter, which is efficiently repressed at high concentrations of glucose. Please consult Badran and Liu, Nature Communications (2015) for more detailed strain maintenance protocols.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MP-P9 was a gift from David Liu (Addgene plasmid # 69668 ; http://n2t.net/addgene:69668 ; RRID:Addgene_69668)
For your References section:Development of potent in vivo mutagenesis plasmids with broad mutational spectra. Badran AH, Liu DR. Nat Commun. 2015 Oct 7;6:8425. doi: 10.1038/ncomms9425. 10.1038/ncomms9425 PubMed 26443021