Beta1-GFP pFB-Neo
(Plasmid #69767)

• Purpose: Expresses Beta1-GFP integrin for retroviral transfection
• Depositing Lab: Martin Humphries
• Publication: Parsons et al J Cell Sci. 2008 Feb 1;121(Pt 3):265-71. doi: 10.1242/jcs.018440.

Backbone

• Vector backbone: pFB-Neo
• Backbone manufacturer: Stratagene
• Backbone size w/o insert (bp): 6600
• Total vector size (bp): 10300
• Vector type: Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Beta1 integrin
• Alt name: ITGB1
• Species: H. sapiens (human)
• Insert Size (bp): 3700
• GenBank ID: NM_002211.3
• Entrez Gene: ITGB1 (a.k.a. CD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB)
• Promoter: IRES
• Tag / Fusion Protein:
  • GFP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoR1 (not destroyed)
• 3′ cloning site: Not1 (not destroyed)
• 5′ sequencing primer: 5´Retro-GGCTGCCGACCCCGGGGGTGG-3´ (bp2008–2028)
• 3′ sequencing primer: 5´-GCCAGGTTTCCGGGCCCTCAC-3´ (bp2147–2127)

Resource Information

• Articles Citing this Plasmid:
  • 2 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry