Beta1-GFP in pHcgreen
(Plasmid #69804)

• Purpose: Expresses Beta1-GFP integrin for normal cell transfection
• Depositing Lab: Martin Humphries
• Publication: Parsons et al J Cell Sci. 2008 Feb 1;121(Pt 3):265-71. doi: 10.1242/jcs.018440.

Backbone

• Vector backbone: pHcRed1-N1/1
• Backbone manufacturer: Clontech
• Backbone size w/o insert (bp): 4700
• Modifications to backbone: after cloning Beta1 into pHcRed the RFp fluorophore sequence was excised usin Age1 and Not1, and the GFP sequence from pEGFPN1 cloned into the pHcRed backbone using the same restriction sites
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Beta1A integrin
• Alt name: ITGB1
• Species: H. sapiens (human)
• Insert Size (bp): 3700
• GenBank ID: NM_002211.3
• Entrez Gene: ITGB1 (a.k.a. CD29, FNRB, GPIIA, MDF2, MSK12, VLA-BETA, VLAB)
• Promoter: CMV
• Tag / Fusion Protein:
  • GFP

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Kpn1 (unknown if destroyed)
• 3′ cloning site: Age1 (unknown if destroyed)
• 5′ sequencing primer: CMV-F
• 3′ sequencing primer: SV40A-R

Resource Information

• Articles Citing this Plasmid:
  • 4 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry