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CRISPRmTmG2
(Plasmid #69992)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 69992 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    gRNA that targets near LoxP sites
  • Insert Size (bp)
    20
  • GenBank ID
    N/A N/A

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Px330 vector was obtained from Addgene (plasmid 42230). gRNA: 5'-CGAAGTTATATTAAGGGTTC 3' was cloned into BbsI site.
  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CRISPRmTmG2 was a gift from Connie Cepko (Addgene plasmid # 69992 ; http://n2t.net/addgene:69992 ; RRID:Addgene_69992)
  • For your References section:

    A gene regulatory network controls the binary fate decision of rod and bipolar cells in the vertebrate retina. Wang S, Sengel C, Emerson MM, Cepko CL. Dev Cell. 2014 Sep 8;30(5):513-27. doi: 10.1016/j.devcel.2014.07.018. Epub 2014 Aug 21. 10.1016/j.devcel.2014.07.018 PubMed 25155555