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pPS22 (pET23a-Cas10/Csm/csm2H6N)
(Plasmid #70038)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 70038 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET23a
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 3666
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Cas10/Csm complex
  • Species
    S. epidermidis
  • Promoter T7
  • Tag / Fusion Protein
    • 6xHIS on N terminus of Csm2

Cloning Information

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pPS22 (pET23a-Cas10/Csm/csm2H6N) was a gift from Luciano Marraffini (Addgene plasmid # 70038)
  • For your References section:

    A ruler protein in a complex for antiviral defense determines the length of small interfering CRISPR RNAs. Hatoum-Aslan A, Samai P, Maniv I, Jiang W, Marraffini LA. J Biol Chem. 2013 Sep 27;288(39):27888-97. doi: 10.1074/jbc.M113.499244. Epub 2013 Aug 9. 10.1074/jbc.M113.499244 PubMed 23935102