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p5E-mfap4
(Plasmid #70052)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 70052 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    p5E-MCS
  • Backbone size w/o insert (bp) 2765
  • Total vector size (bp) 4367
  • Vector type
    Gateway 5' Element

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    mfap4 promoter
  • Species
    D. rerio (zebrafish)
  • Insert Size (bp)
    1602

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer M13F (-21)
  • 3′ sequencing primer M13R
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
  • Industry Terms
    • Not Available to Industry

Depositor Comments

Please note: Addgene's quality control sequencing has identified a number of single-nucleotide discrepancies, compared to the NCBI Genbank reference sequence for the Zebrafish mfap4 promoter. The depositing laboratory is aware of these differences, and states that they are polymorphisms. This plasmid accurately reflects the construct as it was used in the associated publication.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    p5E-mfap4 was a gift from David Tobin (Addgene plasmid # 70052 ; http://n2t.net/addgene:70052 ; RRID:Addgene_70052)
  • For your References section:

    The Macrophage-Specific Promoter mfap4 Allows Live, Long-Term Analysis of Macrophage Behavior during Mycobacterial Infection in Zebrafish. Walton EM, Cronan MR, Beerman RW, Tobin DM. PLoS One. 2015 Oct 7;10(10):e0138949. doi: 10.1371/journal.pone.0138949. eCollection 2015. PONE-D-15-31888 [pii] PubMed 26445458