pMA4008
(Plasmid #70109)

• Purpose: mtDNA destruction/production of rho-0 cells
• Depositing Lab: Mikhail Alexeyev
• Publication: Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi: 10.1371/journal.pone.0154684. eCollection 2016.

Backbone

• Vector backbone: pMA898
• Backbone manufacturer: Alexeyev lab
• Backbone size w/o insert (bp): 4321
• Total vector size (bp): 6767
• Vector type: Mammalian Expression
• Selectable markers: None

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: EGFP
• Insert Size (bp): 720
• Promoter: RSV
• Tag / Fusion Protein:
  • none

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: None
• 3′ sequencing primer: None

Gene/Insert 2

• Gene/Insert name: Herpes simplex virus UL12.5M185
• Alt name: Ul12.5M185
• Species: Herpes Simplex Virus
• Insert Size (bp): 1326
• Mutation: N-terminal deletion of UL12.5
• Promoter: CMV
• Tag / Fusion Protein:
  • none

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: None
• 3′ sequencing primer: None

Resource Information

• Articles Citing this Plasmid:
  • 3 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry