pMA3790
(Plasmid #70110)

• Purpose: mtDNA elimination/production of rho-0 cells
• Depositing Lab: Mikhail Alexeyev
• Publication: Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi: 10.1371/journal.pone.0154684. eCollection 2016.

Backbone

• Vector backbone: pMA2008
• Backbone manufacturer: Alexeyev Lab
• Backbone size w/o insert (bp): 4064
• Total vector size (bp): 7117
• Vector type: Mammalian Expression
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: EGFP
• Insert Size (bp): 720
• Promoter: EF1a
• Tag / Fusion Protein:
  • None

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: None
• 3′ sequencing primer: None

Gene/Insert 2

• Gene/Insert name: hUNG1 Y147A
• Alt name: uracil-N-glycosylase, mitochondrial
• Species: H. sapiens (human)
• Insert Size (bp): 816
• Mutation: Y147A, aa's 1-77 removed
• Promoter: CMV
• Tags / Fusion Proteins:
  • human OTC mitochondrial targeting sequence (N terminal on insert)
  • myc tag (N terminal on insert)

Cloning Information for Gene/Insert 2

• Cloning method: Unknown
• 5′ sequencing primer: None
• 3′ sequencing primer: None

Gene/Insert 3

• Gene/Insert name: Neo
• Alt name: G418 resistance gene, kanamycin resistance gene
• Insert Size (bp): 795
• Promoter: SV40 and native

Cloning Information for Gene/Insert 3

• Cloning method: Unknown
• 5′ sequencing primer: None
• 3′ sequencing primer: None

Resource Information

• Supplemental Documents:
  • 3790 GenBank
• Articles Citing this Plasmid:
  • 6 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry