pLoxP-DHFR-mCherry
(Plasmid #70147)

• Purpose: Expresses a DHFR-mCherry fusion protein and uses for a CRISPR gene deletion
• Depositing Lab: David Sibley
• Publication: Long et al Infect Immun. 2016 Jan 11. doi: 10.1128/IAI.01173-15

Backbone

• Vector backbone: pUC19
• Backbone manufacturer: invitrogen
• Backbone size w/o insert (bp): 2100
• Total vector size (bp): 7317
• Modifications to backbone: NO
• Vector type: Expression in Toxoplasma gondii
• Selectable markers: DHFR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH10B
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: a selectable dihydrofolate reductase-thymidylate synthase marker
• Alt name: DHFR-TS-mCherry
• Species: Toxoplasma gondii
• Insert Size (bp): 4586
• GenBank ID: XM_002367211.1 XM_002367211.1
• Promoter: DHFR 5' UTR
• Tag / Fusion Protein:
  • mCherry (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: M13F
• 3′ sequencing primer: M13R

Resource Information

• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Kevin Brown and Shaojun Long jointly built the plasmid in David Sibley lab, for CRISPR genetic operations.