pRL1124
(Plasmid #70690)

• Purpose: provides avaiM, eco47iiM, and ecot22iM that encode specific methylases to enable transfer of plasmids from E. coli to Anabaena sp. strain PCC 7120 and other strains. lacks a mobilization function.
• Depositing Lab: Peter Wolk
• Publication: Elhai et al J Bacteriol. 1997 Mar;179(6):1998-2005.

Backbone

• Vector backbone: pACYC177
• Backbone manufacturer: ATCC
• Vector type: Bacterial shuttle vector
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha MCR
• Growth instructions: The plasmid must be in appropriately mcr mrr strains such as NEB 10-beta or DH5alpha MCR (or, for the methylases in pRL1124, HB101 will suffice) .
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: avaiM

Gene/Insert 2

• Gene/Insert name: eco47iiM

Gene/Insert 3

• Gene/Insert name: ecot22iM

Resource Information

• A portion of this plasmid was derived from a plasmid made by: We received pACYC177 from the laboratory of Barry Chelm.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry