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pET28-hTDG
(Plasmid #70758)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 70758 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pET28c(+)
  • Backbone manufacturer
    Novagen
  • Backbone size w/o insert (bp) 5367
  • Total vector size (bp) 6553
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    TDG
  • Alt name
    thymine DNA glycosylase
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    1236
  • GenBank ID
    NM_003211.4
  • Entrez Gene
    TDG (a.k.a. hTDG)
  • Promoter T7/lac operon
  • Tag / Fusion Protein
    • 6-HIS (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NheI (not destroyed)
  • 3′ cloning site SalI (not destroyed)
  • 5′ sequencing primer pBRrevBam
  • 3′ sequencing primer T7 terminal
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pET28-hTDG was a gift from Primo Schaer (Addgene plasmid # 70758 ; http://n2t.net/addgene:70758 ; RRID:Addgene_70758)
  • For your References section:

    3CAPS - a structural AP-site analogue as a tool to investigate DNA base excision repair. Schuermann D, Scheidegger SP, Weber AR, Bjoras M, Leumann CJ, Schar P. Nucleic Acids Res. 2016 Jan 4. pii: gkv1520. 10.1093/nar/gkv1520 PubMed 26733580