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Addgene

pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro Notes

Addgene Diagnostic Digest- MluI

This gel depicts the NEB 1kb extend ladder and a digestion of the plasmid pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro with the following enzyme: MluI-HF.

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Addgene Diagnostic Digest- NotI/KpnI

This gel depicts the NEB 1kb ladder and a digestion of the plasmid pLV hU6-sgRNA hUbC-dCas9-KRAB-T2a-Puro with the following enzymes: NotI-HF and KpnI-HF.

We observe the expected two bands: ~8.8kb and 6.1kb

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