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pGM55
(Plasmid #71249)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71249 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pXPG
  • Backbone manufacturer
    Cockerill lab, Addgene plasmid 71248
  • Total vector size (bp) 6188
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    GM-CSF minimal promoter (-55 to +28)
  • Alt name
    colony stimulating factor 2 (granulocyte-macrophage)
  • Species
    H. sapiens (human)
  • Entrez Gene
    CSF2 (a.k.a. CSF, GMCSF)
  • Promoter GM-CSF minimal
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

JM109 are the cells used by the author for pXPG and its derivatives.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGM55 was a gift from Peter Cockerill (Addgene plasmid # 71249 ; http://n2t.net/addgene:71249 ; RRID:Addgene_71249)
  • For your References section:

    Generation of an improved luciferase reporter gene plasmid that employs a novel mechanism for high-copy replication. Bert AG, Burrows J, Osborne CS, Cockerill PN. Plasmid. 2000 Sep;44(2):173-82. 10.1006/plas.2000.1474 PubMed 10964627