|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||71249||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerCockerill lab, Addgene plasmid 71248
- Total vector size (bp) 6188
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameGM-CSF minimal promoter (-55 to +28)
Alt namecolony stimulating factor 2 (granulocyte-macrophage)
SpeciesH. sapiens (human)
Entrez GeneCSF2 (a.k.a. CSF, GMCSF)
- Promoter GM-CSF minimal
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer unknown
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
JM109 are the cells used by the author for pXPG and its derivatives.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pGM55 was a gift from Peter Cockerill (Addgene plasmid # 71249 ; http://n2t.net/addgene:71249 ; RRID:Addgene_71249)
For your References section:Generation of an improved luciferase reporter gene plasmid that employs a novel mechanism for high-copy replication. Bert AG, Burrows J, Osborne CS, Cockerill PN. Plasmid. 2000 Sep;44(2):173-82. 10.1006/plas.2000.1474 PubMed 10964627