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(Plasmid #71256)


Item Catalog # Description Quantity Price (USD)
Plasmid 71256 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Cockerill lab, Addgene plasmid 71248
  • Total vector size (bp) 6297
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    Thymidine Kinase promoter
  • Species
  • Promoter HSV Thymidine Kinase
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sacl (destroyed during cloning)
  • 3′ cloning site PvuII (destroyed during cloning)
  • 5′ sequencing primer unknown
  • 3′ sequencing primer LucNRev
  • (Common Sequencing Primers)

Resource Information

Depositor Comments

JM109 are the cells used by the author for pXPG and its derivatives.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTK229 was a gift from Peter Cockerill (Addgene plasmid # 71256 ; ; RRID:Addgene_71256)
  • For your References section:

    The human IL-3 locus is regulated cooperatively by two NFAT-dependent enhancers that have distinct tissue-specific activities. Hawwari A, Burrows J, Vadas MA, Cockerill PN. J Immunol. 2002 Aug 15;169(4):1876-86. PubMed 12165512