p420-3
(Plasmid #71257)

• Purpose: luciferase reporter with 3 copies of the GM420 NFAT-AP-1 site in front of hGM-CSF -55 to +28 minimal promoter
• Depositing Lab: Peter Cockerill
• Publication: Johnson et al Mol Cell Biol. 2004 Sep;24(18):7914-30.

Backbone

• Vector backbone: pGM55
• Backbone manufacturer: Cockerill lab, Addgene plasmid 71249
• Vector type: Mammalian Expression, Luciferase

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: 3 copies of the GM420 NFAT-AP-1 site
• Species: H. sapiens (human)
• Entrez Gene: CSF2 (a.k.a. CSF, GMCSF)
• Promoter: GM-CSF minimal
• Tag / Fusion Protein:
  • luciferase (C terminal on backbone)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: unknown
• 3′ sequencing primer: LucNRev

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Luciferase Limited Use Label License
• Industry Terms:
  • Not Available to Industry

Depositor Comments

JM109 are the cells used by the author for pXPG and its derivatives.