Purposeluciferase reporter with 3 copies of the GM420 NFAT-AP-1 site in front of hGM-CSF -55 to +28 minimal promoter
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||71257||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerCockerill lab, Addgene plasmid 71249
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name3 copies of the GM420 NFAT-AP-1 site
SpeciesH. sapiens (human)
Entrez GeneCSF2 (a.k.a. CSF, GMCSF)
- Promoter GM-CSF minimal
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer unknown
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
JM109 are the cells used by the author for pXPG and its derivatives.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:p420-3 was a gift from Peter Cockerill (Addgene plasmid # 71257 ; http://n2t.net/addgene:71257 ; RRID:Addgene_71257)
For your References section:Granulocyte-macrophage colony-stimulating factor enhancer activation requires cooperation between NFAT and AP-1 elements and is associated with extensive nucleosome reorganization. Johnson BV, Bert AG, Ryan GR, Condina A, Cockerill PN. Mol Cell Biol. 2004 Sep;24(18):7914-30. 10.1128/MCB.24.18.7914-7930.2004 PubMed 15340054