|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||71258||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Backbone manufacturerCockerill lab, Addgene plasmid 71249
- Total vector size (bp) 6305
Vector typeMammalian Expression, Luciferase
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert name3 copies of an AP-1 site from the human Stromelysin-1 gene
Alt namematrix metallopeptidase 3
SpeciesH. sapiens (human)
Entrez GeneMMP3 (a.k.a. CHDS6, MMP-3, SL-1, STMY, STMY1, STR1)
- Promoter GM-CSF minimal
/ Fusion Protein
- luciferase (C terminal on backbone)
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site SmaI (destroyed during cloning)
- 5′ sequencing primer unknown
- 3′ sequencing primer LucNRev (Common Sequencing Primers)
Cloned as 3 copies of an oligonucleotide duplex with complementary overhanging BglII ends: AGATCTGGATCACCCGCAGCTTGACTCATCCTTGCAGATCT
Reference for AP-1 site = Cockerill PN, Shannon MF, Bert AG, Ryan GR and Vadas MA. The GM-CSF/IL3 locus is regulated by an inducible cyclosporin A sensitive enhancer. Proc.Natl.Acad.Sci. USA 90, 2466-2470, 1993.
JM109 are the cells used by the author for pXPG and its derivatives.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAP1-3 was a gift from Peter Cockerill (Addgene plasmid # 71258 ; http://n2t.net/addgene:71258 ; RRID:Addgene_71258)