pBABEneo-HRASV12
(Plasmid #71304)

• Purpose: Retroviral expression of H-RAS-V12
• Depositing Lab: Jayanta Debnath
• Publication: Lock et al Mol Biol Cell. 2011 Jan 15;22(2):165-78. doi: 10.1091/mbc.E10-06-0500. Epub 2010 Nov 30.

Backbone

• Vector backbone: pBABE-neo
• Backbone size w/o insert (bp): 5300
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: H-RAS-V12
• Alt name: HRAS
• Alt name: HRASV12
• Species: H. sapiens (human)
• Mutation: G12V mutation
• Entrez Gene: HRAS (a.k.a. C-BAS/HAS, C-H-RAS, C-HA-RAS1, CTLO, H-RASIDX, HAMSV, HRAS1, RASH1, p21ras)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: pBABE 5'
• 3′ sequencing primer: pBABE 3'

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

The insert within this plasmid contains a E143K mutation compared to NP_005334.1. The depositor states that this mutation should not affect plasmid function.

The HRAS coding sequence may have been codon optimized and differs from the NCBI reference sequences for this gene. Primer design and applications involving the use of the HRAS coding sequence should be based off of the Addgene NGS Result for accuracy