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pTRK669
(Plasmid #71313)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71313 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGK12
  • Backbone size (bp) 4378
  • Modifications to backbone
    removal of erythromycin resistance gene
  • Vector type
    Bacterial Expression ; E. coli-gram positive bacteria shuttle vector

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH10B
  • Growth instructions
    Chloramphenicol selection is 15 micrograms/mL in E.coli
  • Copy number
    Low Copy

Resource Information

  • Terms and Licenses

Depositor Comments

In plasmid preparations from E. coli, a smaller deletion derivative of pTRK669 of about 1.7 kb may be noticeable. This smaller plasmid does not affect the functionality of pTRK669 in gram positive hosts and may not appear in those backgrounds. Addgene's quality control sequence shows the expected deletion from the smaller derivative of pTRK669.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pTRK669 was a gift from Rodolphe Barrangou & Todd Klaenhammer (Addgene plasmid # 71313 ; http://n2t.net/addgene:71313 ; RRID:Addgene_71313)
  • For your References section:

    Efficient system for directed integration into the Lactobacillus acidophilus and Lactobacillus gasseri chromosomes via homologous recombination. Russell WM, Klaenhammer TR. Appl Environ Microbiol. 2001 Sep;67(9):4361-4. PubMed 11526048