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Grp1-PH pGEX2TK
(Plasmid #71379)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 71379 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGEX-2TK
  • Backbone manufacturer
    Amersham-Pharmacia
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Low Copy

Gene/Insert

  • Gene/Insert name
    Grp1-PH
  • Species
    M. musculus (mouse)
  • Mutation
    Pleckstrin homology (PH) domain; aa 264-391
  • Entrez Gene
    Cyth3 (a.k.a. AI648983, ARNO3, CLM3, Grp1, Pscd3)
  • Promoter tac
  • Tag / Fusion Protein
    • GST (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer pGEX5'
  • 3′ sequencing primer pGEX3'
  • (Common Sequencing Primers)

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Grp1-PH pGEX2TK was a gift from Mark Lemmon (Addgene plasmid # 71379 ; http://n2t.net/addgene:71379 ; RRID:Addgene_71379)
  • For your References section:

    Specificity and promiscuity in phosphoinositide binding by pleckstrin homology domains. Kavran JM, Klein DE, Lee A, Falasca M, Isakoff SJ, Skolnik EY, Lemmon MA. J Biol Chem. 1998 Nov 13;273(46):30497-508. PubMed 9804818