pLenSyn2-EGFP-­OMP25C
(Plasmid #71427)

• Purpose: Lentiviral expression of EGFP-OMP25C. For visualization of neuronal mitochondria.
• Depositing Labs:
• Publication: Kysenius et al PLoS One. 2014 Sep 5;9(9):e107129. doi: 10.1371/journal.pone.0107129. eCollection 2014.

Backbone

• Vector backbone: pLenSyn1
• Backbone manufacturer: modified from pWPT-GFP, Didier Trono, Addgene plasmid 12255
• Total vector size (bp): 8300
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Neomycin (select with G418)

Growth in Bacteria

• Bacterial Resistance(s): Kanamycin, 50 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: OMP25 (172-­‐206)
• Alt name: mitochondrial outer membrane protein-25
• Species: R. norvegicus (rat)
• Mutation: aa 172-206 only
• Entrez Gene: Synj2bp (a.k.a. Omp25)
• Promoter: synapsin
• Tag / Fusion Protein:
  • EGFP (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: BamHI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: EGFP-C
• 3′ sequencing primer: 5'-­AGGAGCAACATAGTTAAGA­‐3'

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry