pCLXSN(GFP)-HA-Slc1a5 (mouse)
(Plasmid #71458)

• Purpose: inducible expression fo Slc1a5 in mammalian cells
• Depositing Lab: Shao-Cong Sun
• Publication: Nakaya et al Immunity. 2014 May 15;40(5):692-705. doi: 10.1016/j.immuni.2014.04.007. Epub 2014 May 1.

Backbone

• Vector backbone: pCLXSN-GFP
• Backbone manufacturer: Obtained from Dr. Inder Verma and modified in Dr. Shao-Cong's lab. Addgene Plasmid #27557
• Backbone size w/o insert (bp): 8000
• Vector type: Mammalian Expression, Retroviral
• Selectable markers: GFP

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Slc1a5
• Species: M. musculus (mouse)
• Insert Size (bp): 1650
• Mutation: A85P, L125F and V384A mutations compared to GenBank reference sequence NM_009201.2
• Entrez Gene: Slc1a5 (a.k.a. AAAT, ASCT2, ATBO, M7V1, M7VS1, R16, RDRC, Slc1a7)
• Promoter: CMV
• Tag / Fusion Protein:
  • HA (N terminal on insert)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: XhoI (not destroyed)
• 5′ sequencing primer: CCT CAC TCC TTC TCT AGG CG (or pLXSN 5')
• 3′ sequencing primer: CAC CCT AAC TGA CAC ACA TTC

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

We transferred the Slc1a5 insert from the pCLXSN vector. The differences in Slc1a5 compared to GenBank reference sequence NM_009201.2 do not affect the function of the plasmid.