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pCLXSN(GFP)-HA-Slc1a5 (mouse)
(Plasmid #71458)

Full plasmid sequence is not available for this item.


Item Catalog # Description Quantity Price (USD)
Plasmid 71458 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
    Obtained from Dr. Inder Verma and modified in Dr. Shao-Cong's lab. Addgene Plasmid #27557
  • Backbone size w/o insert (bp) 8000
  • Vector type
    Mammalian Expression, Retroviral
  • Selectable markers

Growth in Bacteria

  • Bacterial Resistance(s)
  • Growth Temperature
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy


  • Gene/Insert name
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
  • Mutation
    A85P, L125F and V384A mutations compared to GenBank reference sequence NM_009201.2
  • Entrez Gene
    Slc1a5 (a.k.a. AAAT, ASCT2, ATBO, M7V1, M7VS1, R16, RDRC, Slc1a7)
  • Promoter CMV
  • Tag / Fusion Protein
    • HA (N terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site EcoRI (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer CCT CAC TCC TTC TCT AGG CG (or pLXSN 5')
  • 3′ sequencing primer CAC CCT AAC TGA CAC ACA TTC
  • (Common Sequencing Primers)

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

We transferred the Slc1a5 insert from the pCLXSN vector. The differences in Slc1a5 compared to GenBank reference sequence NM_009201.2 do not affect the function of the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCLXSN(GFP)-HA-Slc1a5 (mouse) was a gift from Shao-Cong Sun (Addgene plasmid # 71458 ; ; RRID:Addgene_71458)