pET/ELP-I-CAT
(Plasmid #71461)

• Purpose: (Empty Backbone) ELP-intein purification system
• Depositing Lab: David Wood
• Publication: Banki et al Nat Methods. 2005 Sep;2(9):659-61.

Backbone

• Vector backbone: pET21(+)
• Backbone manufacturer: Novagen
• Modifications to backbone: Added ELP and intein tags
• Vector type: Bacterial Expression
• Tags / Fusion Proteins:
  • ELP ((VPGXG)110) (N terminal on backbone)
  • intein (N terminal on backbone)

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Low Copy

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ sequencing primer: T7 to sequence ELP
• 3′ sequencing primer: F1ori-R

Resource Information

• Supplemental Documents:
  • ELP Cloning Manual
• Addgene Notes:
  • Addgene diagnostic digest

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

This plasmid contains the CAT gene, which can be replaced with your gene of interest using the unique BsrG I site (TGTACA) at the end of the intein and one of several sites downstream from the product protein (typically Hind III). More info on cloning can be found in supplemental document ELP cloning manual.

Please NOTE- the Addgene diagnostic digest result confirms the presence of ELP-intein.