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(Plasmid #71534)


Item Catalog # Description Quantity Price (USD)
Plasmid 71534 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size (bp) 2580
  • Modifications to backbone
    A fragment containing MpU6 promoter (2 kb), CmR-ccdB genes flanked by SacI and PstI sites, and gRNA backbone was inserted by TOPO cloning.
  • Vector type

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol and Kanamycin
  • Growth Temperature
  • Growth Strain(s)
    ccdB Survival
  • Copy number
    High Copy

Cloning Information

  • Cloning method Gibson Cloning

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    Addgene Plasmid #41824
  • Terms and Licenses

Depositor Comments

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMpGE_En01 was a gift from Ikuko Hara-Nishimura & Takayuki Kohchi (Addgene plasmid # 71534 ; ; RRID:Addgene_71534)
  • For your References section:

    Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha. Sugano SS, Nishihama R, Shirakawa M, Takagi J, Matsuda Y, Ishida S, Shimada T, Hara-Nishimura I, Osakabe K, Kohchi T. PLoS One. 2018 Oct 31;13(10):e0205117. doi: 10.1371/journal.pone.0205117. eCollection 2018. 10.1371/journal.pone.0205117 PubMed 30379827