eSpCas9(1.1) Citations (104)
Originally described in: Rationally engineered Cas9 nucleases with improved specificity.Slaymaker IM, Gao L, Zetsche B, Scott DA, Yan WX, Zhang F Science. 2015 Dec 1. pii: aad5227. PubMed Journal
Articles Citing eSpCas9(1.1)
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| A CRISPR Path to Engineering New Genetic Mouse Models for Cardiovascular Research. Miano JM, Zhu QM, Lowenstein CJ. Arterioscler Thromb Vasc Biol. 2016 Jun;36(6):1058-75. doi: 10.1161/ATVBAHA.116.304790. Epub 2016 Apr 21. PubMed |
Probing the impact of chromatin conformation on genome editing tools.
Chen X, Rinsma M, Janssen JM, Liu J, Maggio I, Goncalves MA.
Nucleic Acids Res. 2016 Jun 8. pii: gkw524.
PubMed
Associated Plasmids |
| Comprehensive Protocols for CRISPR/Cas9-based Gene Editing in Human Pluripotent Stem Cells. Santos DP, Kiskinis E, Eggan K, Merkle FT. Curr Protoc Stem Cell Biol. 2016 Aug 17;38:5B.6.1-5B.6.60. doi: 10.1002/cpsc.15. PubMed |
| Experimental Evolution Reveals Interplay between Sch9 and Polyploid Stability in Yeast. Lu YJ, Swamy KB, Leu JY. PLoS Genet. 2016 Nov 3;12(11):e1006409. doi: 10.1371/journal.pgen.1006409. eCollection 2016 Nov. PubMed |
| Epigenetic Enhancement of the Post-replicative DNA Mismatch Repair of Mammalian Genomes by a Hemi-mCpG-Np95-Dnmt1 Axis. Wang KY, Chen CC, Tsai SF, Shen CJ. Sci Rep. 2016 Nov 25;6:37490. doi: 10.1038/srep37490. PubMed |
| Sulforaphane is a Nrf2-independent inhibitor of mitochondrial fission. O'Mealey GB, Berry WL, Plafker SM. Redox Biol. 2016 Nov 21;11:103-110. doi: 10.1016/j.redox.2016.11.007. PubMed |
| Oxidized nucleotide insertion by pol beta confounds ligation during base excision repair. Caglayan M, Horton JK, Dai DP, Stefanick DF, Wilson SH. Nat Commun. 2017 Jan 9;8:14045. doi: 10.1038/ncomms14045. PubMed |
| APC/C Dysfunction Limits Excessive Cancer Chromosomal Instability. Sansregret L, Patterson JO, Dewhurst S, Lopez-Garcia C, Koch A, McGranahan N, Chao WC, Barry DJ, Rowan A, Instrell R, Horswell S, Way M, Howell M, Singleton MR, Medema RH, Nurse P, Petronczki M, Swanton C. Cancer Discov. 2017 Jan 9. doi: 10.1158/2159-8290.CD-16-0645. PubMed |
Practical method for targeted disruption of cilia-related genes by using CRISPR/Cas9-mediated homology-independent knock-in system.
Katoh Y, Michisaka S, Nozaki S, Funabashi T, Hirano T, Takei R, Nakayama K.
Mol Biol Cell. 2017 Feb 8. pii: mbc.E17-01-0051. doi: 10.1091/mbc.E17-01-0051.
PubMed
Associated Plasmids |
Fusion of SpCas9 to E. coli Rec A protein enhances CRISPR-Cas9 mediated gene knockout in mammalian cells.
Lin L, Petersen TS, Jensen KT, Bolund L, Kuhn R, Luo Y.
J Biotechnol. 2017 Mar 1;247:42-49. doi: 10.1016/j.jbiotec.2017.02.024.
PubMed
Associated Plasmids |
| Chromatin accessibility and guide sequence secondary structure affect CRISPR-Cas9 gene editing efficiency. Jensen KT, Floe L, Petersen TS, Huang J, Xu F, Bolund L, Luo Y, Lin L. FEBS Lett. 2017 Jul;591(13):1892-1901. doi: 10.1002/1873-3468.12707. Epub 2017 Jun 28. PubMed |
| Inherited determinants of early recurrent somatic mutations in prostate cancer. Romanel A, Garritano S, Stringa B, Blattner M, Dalfovo D, Chakravarty D, Soong D, Cotter KA, Petris G, Dhingra P, Gasperini P, Cereseto A, Elemento O, Sboner A, Khurana E, Inga A, Rubin MA, Demichelis F. Nat Commun. 2017 Jun 29;8(1):48. doi: 10.1038/s41467-017-00046-0. PubMed |
Rapidly inducible Cas9 and DSB-ddPCR to probe editing kinetics.
Rose JC, Stephany JJ, Valente WJ, Trevillian BM, Dang HV, Bielas JH, Maly DJ, Fowler DM.
Nat Methods. 2017 Jul 24. doi: 10.1038/nmeth.4368.
PubMed
Associated Plasmids |
| Inhibition of post-transcriptional steps in ribosome biogenesis confers cytoprotection against chemotherapeutic agents in a p53-dependent manner. Sapio RT, Nezdyur AN, Krevetski M, Anikin L, Manna VJ, Minkovsky N, Pestov DG. Sci Rep. 2017 Aug 22;7(1):9041. doi: 10.1038/s41598-017-09002-w. PubMed |
| The calcium-binding protein ALG-2 regulates protein secretion and trafficking via interactions with MISSL and MAP1B proteins. Takahara T, Inoue K, Arai Y, Kuwata K, Shibata H, Maki M. J Biol Chem. 2017 Oct 13;292(41):17057-17072. doi: 10.1074/jbc.M117.800201. Epub 2017 Sep 1. PubMed |
CRISPR-Mediated Base Editing Enables Efficient Disruption of Eukaryotic Genes through Induction of STOP Codons.
Billon P, Bryant EE, Joseph SA, Nambiar TS, Hayward SB, Rothstein R, Ciccia A.
Mol Cell. 2017 Sep 4. pii: S1097-2765(17)30605-6. doi: 10.1016/j.molcel.2017.08.008.
PubMed
Associated Plasmids |
Crossing enhanced and high fidelity SpCas9 nucleases to optimize specificity and cleavage.
Kulcsar PI, Talas A, Huszar K, Ligeti Z, Toth E, Weinhardt N, Fodor E, Welker E.
Genome Biol. 2017 Oct 6;18(1):190. doi: 10.1186/s13059-017-1318-8.
PubMed
Associated Plasmids |
| Restoration of Replication Fork Stability in BRCA1- and BRCA2-Deficient Cells by Inactivation of SNF2-Family Fork Remodelers. Taglialatela A, Alvarez S, Leuzzi G, Sannino V, Ranjha L, Huang JW, Madubata C, Anand R, Levy B, Rabadan R, Cejka P, Costanzo V, Ciccia A. Mol Cell. 2017 Oct 19;68(2):414-430.e8. doi: 10.1016/j.molcel.2017.09.036. PubMed |
| TBC1D23 is a bridging factor for endosomal vesicle capture by golgins at the trans-Golgi. Shin JJH, Gillingham AK, Begum F, Chadwick J, Munro S. Nat Cell Biol. 2017 Dec;19(12):1424-1432. doi: 10.1038/ncb3627. Epub 2017 Oct 30. PubMed |
| Fibrinogen Activates BMP Signaling in Oligodendrocyte Progenitor Cells and Inhibits Remyelination after Vascular Damage. Petersen MA, Ryu JK, Chang KJ, Etxeberria A, Bardehle S, Mendiola AS, Kamau-Devers W, Fancy SPJ, Thor A, Bushong EA, Baeza-Raja B, Syme CA, Wu MD, Rios Coronado PE, Meyer-Franke A, Yahn S, Pous L, Lee JK, Schachtrup C, Lassmann H, Huang EJ, Han MH, Absinta M, Reich DS, Ellisman MH, Rowitch DH, Chan JR, Akassoglou K. Neuron. 2017 Dec 6;96(5):1003-1012.e7. doi: 10.1016/j.neuron.2017.10.008. Epub 2017 Nov 2. PubMed |
| Role of Dopamine Receptors in the Anticancer Activity of ONC201. Kline CLB, Ralff MD, Lulla AR, Wagner JM, Abbosh PH, Dicker DT, Allen JE, El-Deiry WS. Neoplasia. 2018 Jan;20(1):80-91. doi: 10.1016/j.neo.2017.10.002. Epub 2017 Dec 5. PubMed |
| CRISPR/Cas9-mediated modulation of splicing efficiency reveals short splicing isoform of Xist RNA is sufficient to induce X-chromosome inactivation. Yue M, Ogawa Y. Nucleic Acids Res. 2018 Mar 16;46(5):e26. doi: 10.1093/nar/gkx1227. PubMed |
| Distinct SoxB1 networks are required for naive and primed pluripotency. Corsinotti A, Wong FC, Tatar T, Szczerbinska I, Halbritter F, Colby D, Gogolok S, Pantier R, Liggat K, Mirfazeli ES, Hall-Ponsele E, Mullin NP, Wilson V, Chambers I. Elife. 2017 Dec 19;6. pii: 27746. doi: 10.7554/eLife.27746. PubMed |
A highly specific SpCas9 variant is identified by in vivo screening in yeast.
Casini A, Olivieri M, Petris G, Montagna C, Reginato G, Maule G, Lorenzin F, Prandi D, Romanel A, Demichelis F, Inga A, Cereseto A.
Nat Biotechnol. 2018 Jan 29. pii: nbt.4066. doi: 10.1038/nbt.4066.
PubMed
Associated Plasmids |
| CRISPR/Cas9 Mediated GFP Knock-in at the MAP1LC3B Locus in 293FT Cells Is Better for Bona Fide Monitoring Cellular Autophagy. Wu Z, Zhao J, Qiu M, Mi Z, Meng M, Guo Y, Wang H, Yuan Z. Biotechnol J. 2018 Nov;13(11):e1700674. doi: 10.1002/biot.201700674. Epub 2018 May 27. PubMed |
| The atypical mitogen-activated protein kinase ERK3 is essential for establishment of epithelial architecture. Takahashi C, Miyatake K, Kusakabe M, Nishida E. J Biol Chem. 2018 Jun 1;293(22):8342-8361. doi: 10.1074/jbc.RA117.000992. Epub 2018 Apr 19. PubMed |
| Retrograde transport of gamma-secretase from endosomes to the trans-Golgi network regulates Abeta42 production. Kanatsu K, Hori Y, Ebinuma I, Chiu YW, Tomita T. J Neurochem. 2018 Oct;147(1):110-123. doi: 10.1111/jnc.14477. Epub 2018 Aug 16. PubMed |
| CRISPR/Cas9-Mediated Genome Editing for Huntington's Disease. Vachey G, Deglon N. Methods Mol Biol. 2018;1780:463-481. doi: 10.1007/978-1-4939-7825-0_21. PubMed |
Esrrb Unlocks Silenced Enhancers for Reprogramming to Naive Pluripotency.
Adachi K, Kopp W, Wu G, Heising S, Greber B, Stehling M, Arauzo-Bravo MJ, Boerno ST, Timmermann B, Vingron M, Scholer HR.
Cell Stem Cell. 2018 Aug 2;23(2):266-275.e6. doi: 10.1016/j.stem.2018.05.020. Epub 2018 Jun 14.
PubMed
Associated Plasmids |
| Functional Dissection of the Enhancer Repertoire in Human Embryonic Stem Cells. Barakat TS, Halbritter F, Zhang M, Rendeiro AF, Perenthaler E, Bock C, Chambers I. Cell Stem Cell. 2018 Aug 2;23(2):276-288.e8. doi: 10.1016/j.stem.2018.06.014. Epub 2018 Jul 19. PubMed |
| Inhibition of Cyclin-Dependent Kinase 5: A Strategy to Improve Sorafenib Response in Hepatocellular Carcinoma Therapy. Ardelt MA, Frohlich T, Martini E, Muller M, Kanitz V, Atzberger C, Cantonati P, Messner M, Posselt L, Lehr T, Wojtyniak JG, Ulrich M, Arnold GJ, Konig L, Parazzoli D, Zahler S, Rothenfusser S, Mayr D, Gerbes A, Scita G, Vollmar AM, Pachmayr J. Hepatology. 2019 Jan;69(1):376-393. doi: 10.1002/hep.30190. Epub 2018 Dec 22. PubMed |
| Heterologous Hsp90 promotes phenotypic diversity through network evolution. Koubkova-Yu TC, Chao JC, Leu JY. PLoS Biol. 2018 Nov 15;16(11):e2006450. doi: 10.1371/journal.pbio.2006450. eCollection 2018 Nov. PubMed |
| Predicting the mutations generated by repair of Cas9-induced double-strand breaks. Allen F, Crepaldi L, Alsinet C, Strong AJ, Kleshchevnikov V, De Angeli P, Palenikova P, Khodak A, Kiselev V, Kosicki M, Bassett AR, Harding H, Galanty Y, Munoz-Martinez F, Metzakopian E, Jackson SP, Parts L. Nat Biotechnol. 2018 Nov 27. pii: nbt.4317. doi: 10.1038/nbt.4317. PubMed |
| An alternative membrane topology permits lipid droplet localization of peroxisomal fatty acyl-CoA reductase 1. Exner T, Romero-Brey I, Yifrach E, Rivera-Monroy J, Schrul B, Zouboulis CC, Stremmel W, Honsho M, Bartenschlager R, Zalckvar E, Poppelreuther M, Fullekrug J. J Cell Sci. 2019 Mar 18;132(6). pii: jcs.223016. doi: 10.1242/jcs.223016. PubMed |
Loss of Emp2 compromises cardiogenic differentiation in mouse embryonic stem cells.
Liu Y, Dakou E, Meng Y, Leyns L.
Biochem Biophys Res Commun. 2019 Feb 14. pii: S0006-291X(19)30235-9. doi: 10.1016/j.bbrc.2019.02.048.
PubMed
Associated Plasmids |
| Selective Roles of Vertebrate PCF11 in Premature and Full-Length Transcript Termination. Kamieniarz-Gdula K, Gdula MR, Panser K, Nojima T, Monks J, Wisniewski JR, Riepsaame J, Brockdorff N, Pauli A, Proudfoot NJ. Mol Cell. 2019 Apr 4;74(1):158-172.e9. doi: 10.1016/j.molcel.2019.01.027. Epub 2019 Feb 25. PubMed |
| Acute Inhibition of Heterotrimeric Kinesin-2 Function Reveals Mechanisms of Intraflagellar Transport in Mammalian Cilia. Engelke MF, Waas B, Kearns SE, Suber A, Boss A, Allen BL, Verhey KJ. Curr Biol. 2019 Apr 1;29(7):1137-1148.e4. doi: 10.1016/j.cub.2019.02.043. Epub 2019 Mar 21. PubMed |
| Boosting activity of high-fidelity CRISPR/Cas9 variants using a tRNA(Gln)-processing system in human cells. He X, Wang Y, Yang F, Wang B, Xie H, Gu L, Zhao T, Liu X, Zhang D, Ren Q, Liu X, Liu Y, Gao C, Gu F. J Biol Chem. 2019 Jun 7;294(23):9308-9315. doi: 10.1074/jbc.RA119.007791. Epub 2019 Apr 22. PubMed |
| Functional evaluation of transposable elements as enhancers in mouse embryonic and trophoblast stem cells. Todd CD, Deniz O, Taylor D, Branco MR. Elife. 2019 Apr 23;8. pii: 44344. doi: 10.7554/eLife.44344. PubMed |
| Genome Editing in Mammalian Cell Lines using CRISPR-Cas. Liu KI, Sutrisnoh NB, Wang Y, Tan MH. J Vis Exp. 2019 Apr 11;(146). doi: 10.3791/59086. PubMed |
| Caspase-1 initiates apoptosis in the absence of gasdermin D. Tsuchiya K, Nakajima S, Hosojima S, Thi Nguyen D, Hattori T, Manh Le T, Hori O, Mahib MR, Yamaguchi Y, Miura M, Kinoshita T, Kushiyama H, Sakurai M, Shiroishi T, Suda T. Nat Commun. 2019 May 7;10(1):2091. doi: 10.1038/s41467-019-09753-2. PubMed |
| Possible detrimental effects of beta-2-microglobulin knockout in pigs. Sake HJ, Frenzel A, Lucas-Hahn A, Nowak-Imialek M, Hassel P, Hadeler KG, Hermann D, Becker R, Eylers H, Hein R, Baars W, Brinkmann A, Schwinzer R, Niemann H, Petersen B. Xenotransplantation. 2019 May 22:e12525. doi: 10.1111/xen.12525. PubMed |
| Single-Molecule Nanoscopy Elucidates RNA Polymerase II Transcription at Single Genes in Live Cells. Li J, Dong A, Saydaminova K, Chang H, Wang G, Ochiai H, Yamamoto T, Pertsinidis A. Cell. 2019 Jul 11;178(2):491-506.e28. doi: 10.1016/j.cell.2019.05.029. Epub 2019 May 30. PubMed |
| TRAF6 maintains mammary stem cells and promotes pregnancy-induced mammary epithelial cell expansion. Yamamoto M, Abe C, Wakinaga S, Sakane K, Yumiketa Y, Taguchi Y, Matsumura T, Ishikawa K, Fujimoto J, Semba K, Miyauchi M, Akiyama T, Inoue JI. Commun Biol. 2019 Aug 6;2:292. doi: 10.1038/s42003-019-0547-7. eCollection 2019. PubMed |
| Off-target effects of CRISPRa on interleukin-6 expression. Soubeyrand S, Lau P, Peters V, McPherson R. PLoS One. 2019 Oct 28;14(10):e0224113. doi: 10.1371/journal.pone.0224113. eCollection 2019. PubMed |
| CD146 Regulates Growth Factor-Induced mTORC2 Activity Independent of the PI3K and mTORC1 Pathways. Xu W, Hua H, Chiu YH, Li G, Zhi H, Yu Z, Ren F, Luo Y, Cui W. Cell Rep. 2019 Oct 29;29(5):1311-1322.e5. doi: 10.1016/j.celrep.2019.09.047. PubMed |
| Trans-Synaptic Signaling through the Glutamate Receptor Delta-1 Mediates Inhibitory Synapse Formation in Cortical Pyramidal Neurons. Fossati M, Assendorp N, Gemin O, Colasse S, Dingli F, Arras G, Loew D, Charrier C. Neuron. 2019 Dec 18;104(6):1081-1094.e7. doi: 10.1016/j.neuron.2019.09.027. Epub 2019 Nov 5. PubMed |
| Transcription factor binding at Ig enhancers is linked to somatic hypermutation targeting. Dinesh RK, Barnhill B, Ilanges A, Wu L, Michelson DA, Senigl F, Alinikula J, Shabanowitz J, Hunt DF, Schatz DG. Eur J Immunol. 2020 Mar;50(3):380-395. doi: 10.1002/eji.201948357. Epub 2019 Dec 19. PubMed |
The E3 Ubiquitin Ligase Mind Bomb 1 Controls Adenovirus Genome Release at the Nuclear Pore Complex.
Bauer M, Flatt JW, Seiler D, Cardel B, Emmenlauer M, Boucke K, Suomalainen M, Hemmi S, Greber UF.
Cell Rep. 2019 Dec 17;29(12):3785-3795.e8. doi: 10.1016/j.celrep.2019.11.064.
PubMed
Associated Plasmids |
| Genome Editing in Patient iPSCs Corrects the Most Prevalent USH2A Mutations and Reveals Intriguing Mutant mRNA Expression Profiles. Sanjurjo-Soriano C, Erkilic N, Baux D, Mamaeva D, Hamel CP, Meunier I, Roux AF, Kalatzis V. Mol Ther Methods Clin Dev. 2019 Nov 27;17:156-173. doi: 10.1016/j.omtm.2019.11.016. eCollection 2020 Jun 12. PubMed |
| Naturally occurring NOTCH3 exon skipping attenuates NOTCH3 protein aggregation and disease severity in CADASIL patients. Gravesteijn G, Dauwerse JG, Overzier M, Brouwer G, Hegeman I, Mulder AA, Baas F, Kruit MC, Terwindt GM, van Duinen SG, Jost CR, Aartsma-Rus A, Lesnik Oberstein SAJ, Rutten JW. Hum Mol Genet. 2020 Jul 21;29(11):1853-1863. doi: 10.1093/hmg/ddz285. PubMed |
Blackjack mutations improve the on-target activities of increased fidelity variants of SpCas9 with 5'G-extended sgRNAs.
Kulcsar PI, Talas A, Toth E, Nyeste A, Ligeti Z, Welker Z, Welker E.
Nat Commun. 2020 Mar 6;11(1):1223. doi: 10.1038/s41467-020-15021-5.
PubMed
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| FAM111A protects replication forks from protein obstacles via its trypsin-like domain. Kojima Y, Machida Y, Palani S, Caulfield TR, Radisky ES, Kaufmann SH, Machida YJ. Nat Commun. 2020 Mar 12;11(1):1318. doi: 10.1038/s41467-020-15170-7. PubMed |
| Suppression of unwanted CRISPR-Cas9 editing by co-administration of catalytically inactivating truncated guide RNAs. Rose JC, Popp NA, Richardson CD, Stephany JJ, Mathieu J, Wei CT, Corn JE, Maly DJ, Fowler DM. Nat Commun. 2020 Jun 1;11(1):2697. doi: 10.1038/s41467-020-16542-9. PubMed |
| Phosphorylation of steroid receptor coactivator-3 (SRC-3) at serine 857 is regulated by the p38(MAPK)-MK2 axis and affects NF-kappaB-mediated transcription. Shrestha A, Bruckmueller H, Kildalsen H, Kaur G, Gaestel M, Wetting HL, Mikkola I, Seternes OM. Sci Rep. 2020 Jul 9;10(1):11388. doi: 10.1038/s41598-020-68219-4. PubMed |
| Endogenous retroviruses are a source of enhancers with oncogenic potential in acute myeloid leukaemia. Deniz O, Ahmed M, Todd CD, Rio-Machin A, Dawson MA, Branco MR. Nat Commun. 2020 Jul 14;11(1):3506. doi: 10.1038/s41467-020-17206-4. PubMed |
| Resection and repair of a Cas9 double-strand break at CTG trinucleotide repeats induces local and extensive chromosomal deletions. Mosbach V, Viterbo D, Descorps-Declere S, Poggi L, Vaysse-Zinkhofer W, Richard GF. PLoS Genet. 2020 Jul 16;16(7):e1008924. doi: 10.1371/journal.pgen.1008924. eCollection 2020 Jul. PubMed |
| Evolutionary divergence reveals the molecular basis of EMRE dependence of the human MCU. MacEwen MJ, Markhard AL, Bozbeyoglu M, Bradford F, Goldberger O, Mootha VK, Sancak Y. Life Sci Alliance. 2020 Aug 7;3(10). pii: 3/10/e202000718. doi: 10.26508/lsa.202000718. Print 2020 Oct. PubMed |
| Negative Regulation of the Differentiation of Flk2(-) CD34(-) LSK Hematopoietic Stem Cells by EKLF/KLF1. Hung CH, Wang KY, Liou YH, Wang JP, Huang AY, Lee TL, Jiang ST, Liao NS, Shyu YC, Shen CJ. Int J Mol Sci. 2020 Nov 10;21(22). pii: ijms21228448. doi: 10.3390/ijms21228448. PubMed |
| MAGE-A3 is a prognostic biomarker for poor clinical outcome in cutaneous squamous cell carcinoma with perineural invasion via modulation of cell proliferation. Chen A, Santana AL, Doudican N, Roudiani N, Laursen K, Therrien JP, Lee J, Felsen D, Carucci JA. PLoS One. 2020 Nov 23;15(11):e0241551. doi: 10.1371/journal.pone.0241551. eCollection 2020. PubMed |
| Generation of PRMT6 homozygous knockout human embryonic stem cell lines. Li T, Jin W, He Y, Zhou Z. Stem Cell Res. 2020 Dec 28;50:102136. doi: 10.1016/j.scr.2020.102136. PubMed |
| The Major Ciliary Isoforms of RPGR Build Different Interaction Complexes with INPP5E and RPGRIP1L. Vossing C, Atigbire P, Eilers J, Markus F, Stieger K, Song F, Neidhardt J. Int J Mol Sci. 2021 Mar 30;22(7). pii: ijms22073583. doi: 10.3390/ijms22073583. PubMed |
| Silencing of LINE-1 retrotransposons is a selective dependency of myeloid leukemia. Gu Z, Liu Y, Zhang Y, Cao H, Lyu J, Wang X, Wylie A, Newkirk SJ, Jones AE, Lee M Jr, Botten GA, Deng M, Dickerson KE, Zhang CC, An W, Abrams JM, Xu J. Nat Genet. 2021 May;53(5):672-682. doi: 10.1038/s41588-021-00829-8. Epub 2021 Apr 8. PubMed |
| Defective Interfering Genomes and the Full-Length Viral Genome Trigger RIG-I After Infection With Vesicular Stomatitis Virus in a Replication Dependent Manner. Linder A, Bothe V, Linder N, Schwarzlmueller P, Dahlstrom F, Bartenhagen C, Dugas M, Pandey D, Thorn-Seshold J, Boehmer DFR, Koenig LM, Kobold S, Schnurr M, Raedler J, Spielmann G, Karimzadeh H, Schmidt A, Endres S, Rothenfusser S. Front Immunol. 2021 Apr 30;12:595390. doi: 10.3389/fimmu.2021.595390. eCollection 2021. PubMed |
| Heterozygous OAS1 gain-of-function variants cause an autoinflammatory immunodeficiency. Magg T, Okano T, Koenig LM, Boehmer DFR, Schwartz SL, Inoue K, Heimall J, Licciardi F, Ley-Zaporozhan J, Ferdman RM, Caballero-Oteyza A, Park EN, Calderon BM, Dey D, Kanegane H, Cho K, Montin D, Reiter K, Griese M, Albert MH, Rohlfs M, Gray P, Walz C, Conn GL, Sullivan KE, Klein C, Morio T, Hauck F. Sci Immunol. 2021 Jun 18;6(60). pii: 6/60/eabf9564. doi: 10.1126/sciimmunol.abf9564. PubMed |
| OAS1/RNase L executes RIG-I ligand-dependent tumor cell apoptosis. Boehmer DFR, Formisano S, de Oliveira Mann CC, Mueller SA, Kluge M, Metzger P, Rohlfs M, Horth C, Kocheise L, Lichtenthaler SF, Hopfner KP, Endres S, Rothenfusser S, Friedel CC, Duewell P, Schnurr M, Koenig LM. Sci Immunol. 2021 Jul 16;6(61). pii: 6/61/eabe2550. doi: 10.1126/sciimmunol.abe2550. PubMed |
| The SARS-CoV-2 Nsp3 macrodomain reverses PARP9/DTX3L-dependent ADP-ribosylation induced by interferon signaling. Russo LC, Tomasin R, Matos IA, Manucci AC, Sowa ST, Dale K, Caldecott KW, Lehtio L, Schechtman D, Meotti FC, Bruni-Cardoso A, Hoch NC. J Biol Chem. 2021 Aug 4;297(3):101041. doi: 10.1016/j.jbc.2021.101041. PubMed |
| Quantitative assessment of engineered Cas9 variants for target specificity enhancement by single-molecule reaction pathway analysis. Bak SY, Jung Y, Park J, Sung K, Jang HK, Bae S, Kim SK. Nucleic Acids Res. 2021 Nov 8;49(19):11312-11322. doi: 10.1093/nar/gkab858. PubMed |
| Loss of Resf1 reduces the efficiency of embryonic stem cell self-renewal and germline entry. Vojtek M, Chambers I. Life Sci Alliance. 2021 Oct 4;4(12). pii: 4/12/e202101190. doi: 10.26508/lsa.202101190. Print 2021 Dec. PubMed |
A functional genetic toolbox for human tissue-derived organoids.
Sun D, Evans L, Perrone F, Sokleva V, Lim K, Rezakhani S, Lutolf M, Zilbauer M, Rawlins EL.
Elife. 2021 Oct 6;10. pii: 67886. doi: 10.7554/eLife.67886.
PubMed
Associated Plasmids |
Multiplex Gene Tagging with CRISPR-Cas9 for Live-Cell Microscopy and Application to Study the Role of SARS-CoV-2 Proteins in Autophagy, Mitochondrial Dynamics, and Cell Growth.
Perez-Leal O, Nixon-Abell J, Barrero CA, Gordon JC, Oesterling J, Rico MC.
CRISPR J. 2021 Nov 30. doi: 10.1089/crispr.2021.0041.
PubMed
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| SARS-CoV-2 infection of olfactory epithelial cells and neurons drives acute lung injury and lethal COVID-19 in mice. Tang AT, Buchholz DW, Szigety KM, Imbhiaka B, Gao S, Frankfurter M, Wang M, Yang J, Hewins P, Mericko-Ishizuka P, Adrian Leu N, Sterling S, Monreal IA, Sahler J, August A, Zhu X, Jurado KA, Xu M, Morrisey EE, Millar SE, Aguilar HC, Kahn ML. bioRxiv. 2021 Dec 7. doi: 10.1101/2021.12.04.471245. PubMed |
Loss of Integrin-linked kinase sensitizes breast cancer to SRC inhibitors.
Beetham H, Griffith BGC, Murina O, Loftus AE, Parry DA, Temps C, Culley J, Muir M, Unciti-Broceta A, Sims AH, Byron A, Brunton VG.
Cancer Res. 2021 Dec 17. pii: 0008-5472.CAN-21-0373. doi: 10.1158/0008-5472.CAN-21-0373.
PubMed
Associated Plasmids |
| Time-controlled and muscle-specific CRISPR/Cas9-mediated deletion of CTG-repeat expansion in the DMPK gene. Cardinali B, Provenzano C, Izzo M, Voellenkle C, Battistini J, Strimpakos G, Golini E, Mandillo S, Scavizzi F, Raspa M, Perfetti A, Baci D, Lazarevic D, Garcia-Manteiga JM, Gourdon G, Martelli F, Falcone G. Mol Ther Nucleic Acids. 2021 Nov 29;27:184-199. doi: 10.1016/j.omtn.2021.11.024. eCollection 2022 Mar 8. PubMed |
| Rapid and robust derivation of mesenchymal stem cells from human pluripotent stem cells via temporal induction of neuralized ectoderm. Jin W, He Y, Li T, Long F, Qin X, Yuan Y, Gao G, Shakhawat HM, Liu X, Jin G, Zhou Z. Cell Biosci. 2022 Mar 15;12(1):31. doi: 10.1186/s13578-022-00753-2. PubMed |
| ABCA1 Expression Is Upregulated in an EMT in Breast Cancer Cell Lines via MYC-Mediated De-Repression of Its Proximal Ebox Element. Prijic S, Chang JT. Biomedicines. 2022 Mar 2;10(3). pii: biomedicines10030581. doi: 10.3390/biomedicines10030581. PubMed |
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