pMES140
(Plasmid
#71849)
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PurposeComplementation of NME1 gene disruption or mutation in yeast
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Depositing Labs
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 71849 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepRS315
- Total vector size (bp) 7000
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Vector typeYeast Expression
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Selectable markersLEU2
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameNME1
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SpeciesS. cerevisiae (budding yeast)
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Entrez GeneNME1 (a.k.a. YNCN0013W)
- Promoter NME1
Cloning Information
- Cloning method Unknown
- 5′ sequencing primer M13-F20
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
CEN, LEU2 based plasmid with NME1 gene region sufficient for NME1 expression in yeast where NME1 gene is disrupted.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pMES140 was a gift from David Clayton & Mark Schmitt (Addgene plasmid # 71849 ; http://n2t.net/addgene:71849 ; RRID:Addgene_71849) -
For your References section:
Nuclear RNase MRP is required for correct processing of pre-5.8S rRNA in Saccharomyces cerevisiae. Schmitt ME, Clayton DA. Mol Cell Biol. 1993 Dec;13(12):7935-41. PubMed 8247008
