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pCORE-Hp53
(Plasmid #72242)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 72242 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFA6aKlURA3
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    p53
  • Alt name
    cDNA of human p53 gene with the V122A mutation
  • Species
    H. sapiens (human)
  • Mutation
    V122A mutation
  • Entrez Gene
    TP53 (a.k.a. BCC7, BMFS5, LFS1, P53, TRP53)

Gene/Insert 2

  • Gene/Insert name
    hyg
  • Alt name
    provides resistance to hygromycin

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This plasmid was created by Francesca Storici.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Resnik and Storici Labs recommend reading the following review articles for more information on this plasmid:
- Storici and Resnick Method Enzymol 2006 PMID: 16793410
- Stuckey et al., Meth. Mol. Biol. 2011 PMID: 21660695
- Stuckey and Storici Methods Enzymol 2013 PMID: 24182920

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCORE-Hp53 was a gift from Michael Resnick & Francesca Storici (Addgene plasmid # 72242 ; http://n2t.net/addgene:72242 ; RRID:Addgene_72242)
  • For your References section:

    Delitto perfetto targeted mutagenesis in yeast with oligonucleotides. Storici F, Resnick MA. Genet Eng (N Y). 2003;25:189-207. PubMed 15260239