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pGSHU
(Plasmid #72244)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 72244 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pFA6aKlURA3
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert 1

  • Gene/Insert name
    KlURA3
  • Alt name
    URA3 gene from Kluyveromyces lactis

Gene/Insert 2

  • Gene/Insert name
    hyg
  • Alt name
    provides resistance to hygromycin

Gene/Insert 3

  • Gene/Insert name
    I-SceI
  • Alt name
    I-SceI endonuclease
  • Species
    S. cerevisiae (budding yeast)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    This plasmid was created by Francesca Storici.

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

The Resnik and Storici Labs recommend reading the following review articles for more information on this plasmid:
- Storici and Resnick Method Enzymol 2006 PMID: 16793410
- Stuckey et al., Meth. Mol. Biol. 2011 PMID: 21660695
- Stuckey and Storici Methods Enzymol 2013 PMID: 24182920

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGSHU was a gift from Michael Resnick & Francesca Storici (Addgene plasmid # 72244 ; http://n2t.net/addgene:72244 ; RRID:Addgene_72244)
  • For your References section:

    Chromosomal site-specific double-strand breaks are efficiently targeted for repair by oligonucleotides in yeast. Storici F, Durham CL, Gordenin DA, Resnick MA. Proc Natl Acad Sci U S A. 2003 Dec 9;100(25):14994-9. Epub 2003 Nov 20. 10.1073/pnas.2036296100 PubMed 14630945