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pCI SMN2
(Plasmid #72287)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 72287 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pCI
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 5472
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SMN2
  • Alt name
    survival of motor neuron 2, centromeric
  • Species
    H. sapiens (human)
  • Insert Size (bp)
    6840
  • Mutation
    contains exons 6-7-8 including introns, multiple SNPs compared to NG_008728.1
  • Entrez Gene
    SMN2 (a.k.a. BCD541, C-BCD541, GEMIN1, SMNC, TDRD16B)
  • Promoter CMV

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site SmaI (not destroyed)
  • 5′ sequencing primer chim-int-F
  • 3′ sequencing primer EBV-rev
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Please note that the Addgene verified sequence detects multiple SNPs compared to the NCBI reference #NG_008728.1. The depositor confirmed that these mutations should not affect plasmid function.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCI SMN2 was a gift from Elliot Androphy (Addgene plasmid # 72287 ; http://n2t.net/addgene:72287 ; RRID:Addgene_72287)
  • For your References section:

    A single nucleotide in the SMN gene regulates splicing and is responsible for spinal muscular atrophy. Lorson CL, Hahnen E, Androphy EJ, Wirth B. Proc Natl Acad Sci U S A. 1999 May 25;96(11):6307-11. PubMed 10339583