pCAG-PuroDTK.Neo
(Plasmid #72307)

• Purpose: PiggyBac Cassette contains puromycin and TK genes
• Depositing Lab: Yuet Wai Kan
• Publication: Ye et al Proc Natl Acad Sci U S A. 2014 Jul 1;111(26):9591-6. doi: 10.1073/pnas.1407473111. Epub 2014 Jun 9.

Backbone

• Vector backbone: 5'-PTK-3'
• Backbone manufacturer: Wellcome trust Sanger Institute
• Backbone size w/o insert (bp): 6376
• Total vector size (bp): 9339
• Modifications to backbone: Insert a CAG promoter, EM7 driven Neo gene
• Vector type: Mammalian Expression, Bacterial Expression
• Selectable markers: Neomycin (select with G418), Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): DH5alpha
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Puromycin-DTK-neo
• Promoter: CAG promoter

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: T7:taatacgactcactatagg
• 3′ sequencing primer: T3: aattaaccctcactaaagg

Resource Information

• Supplemental Documents:
  • Protocol for making donor plasmid
• A portion of this plasmid was derived from a plasmid made by: The backbone plasmid is from Dr. Allan Bradly
• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • piggyBac Limited Use Label License
• Industry Terms:
  • Not Available to Industry