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PurposeDefective Helper to produce neurotropic, propagation-incompetent Sindbis virus
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Depositing Lab
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Publication
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 72309 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSIN
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Vector typeSindbis virus helper construct
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberHigh Copy
Gene/Insert
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Gene/Insert nameDH-BB(5'SIN;TE12ORF)
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SpeciesSindbis virus
Cloning Information
- Cloning method Gibson Cloning
- 5′ sequencing primer TAA AGC ATC TCT ACG GTG GTC C
- 3′ sequencing primer CAT CGA GTT TTG CTG GTC GGA (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byBackbone of this plasmid originates from DH-BB(5'SIN) (generous gift form the Rice lab, Rockefeller University); insert originates from DH(26S)5'SIN (gift of the Malinow lab, UCSD)
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Articles Citing this Plasmid
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
DH-BB(5'SIN;TE12ORF) was a gift from Anthony Zador (Addgene plasmid # 72309 ; http://n2t.net/addgene:72309 ; RRID:Addgene_72309)
