pSuper-PACSIN1-shRNA
(Plasmid
#72576)
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PurposeExpresses shRNA against PACSIN1
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Depositing Labs
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 72576 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSuper
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Backbone manufacturerOligoengine
- Backbone size w/o insert (bp) 3176
- Total vector size (bp) 3208
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Vector typeMammalian Expression, RNAi
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Copy numberUnknown
Gene/Insert
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Gene/Insert namePACSIN1
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gRNA/shRNA sequenceGCGCCAGCTCATCGAGAAA
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SpeciesM. musculus (mouse)
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Entrez GenePacsin1 (a.k.a. A830061D09Rik, H74, mKIAA1379, syndapin)
- Promoter H1 promoter
Cloning Information
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (destroyed during cloning)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer AATACGACTCACTATAG
- 3′ sequencing primer CTTTAGTGAGGGTTAAT (Common Sequencing Primers)
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSuper-PACSIN1-shRNA was a gift from Victor Anggono & Richard Huganir (Addgene plasmid # 72576 ; http://n2t.net/addgene:72576 ; RRID:Addgene_72576) -
For your References section:
PICK1 interacts with PACSIN to regulate AMPA receptor internalization and cerebellar long-term depression. Anggono V, Koc-Schmitz Y, Widagdo J, Kormann J, Quan A, Chen CM, Robinson PJ, Choi SY, Linden DJ, Plomann M, Huganir RL. Proc Natl Acad Sci U S A. 2013 Aug 20;110(34):13976-81. doi: 10.1073/pnas.1312467110. Epub 2013 Aug 5. 10.1073/pnas.1312467110 PubMed 23918399
