tet-pLKO shGATA6 puro
(Plasmid #72615)

• Purpose: Expresses an inducible short hairpin targeting human GATA6 sequence
• Depositing Lab: Kevin Janes
• Publication: Chitforoushzadeh et al Sci Signal. 2016 Jun 7;9(431):ra59. doi: 10.1126/scisignal.aad3373.

Backbone

• Vector backbone: Tet PLKO puro
• Backbone manufacturer: Plasmid #21915
• Backbone size w/o insert (bp): 10633
• Vector type: Mammalian Expression, Lentiviral
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 30°C
• Growth Strain(s): NEB Stable
• Copy number: Low Copy

Gene/Insert

• Gene/Insert name: short hairpin GATA binding protein 6
• Alt name: shGATA6
• gRNA/shRNA sequence: CCCAGACCACTTGCTATGAAA
• Species: H. sapiens (human)
• GenBank ID: NM_005257
• Promoter: TRE Tight
• Tag / Fusion Protein:
  • None

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: AgeI (not destroyed)
• 3′ cloning site: ECORI (not destroyed)
• 5′ sequencing primer: GGCAGGGATATTCACCATTATCGTTTCAGA

Resource Information

• Article Citing this Plasmid:
  • 1 Reference

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry