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pGL4.10 Dub3
(Plasmid #72684)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 72684 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pGL4.10[luc]
  • Backbone manufacturer
    Promega
  • Backbone size w/o insert (bp) 4242
  • Vector type
    Mammalian Expression, Luciferase

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Dub3 promoter
  • Alt name
    ubiquitin specific peptidase 17-like E
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    3200
  • Entrez Gene
    Usp17le (a.k.a. DUB-3, Dub3, Dub6, EG625530, Gm6596, USP17, USP17-E, Usp17l2)
  • Promoter Dub3
  • Tag / Fusion Protein
    • luciferase (C terminal on backbone)

Cloning Information

Terms and Licenses

Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Addgene NGS found a small number of single nucleotide discrepancies relative to Sanger sequencing results provided by the depositing laboratory. These do not affect plasmid function

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pGL4.10 Dub3 was a gift from Domenico Maiorano (Addgene plasmid # 72684 ; http://n2t.net/addgene:72684 ; RRID:Addgene_72684)
  • For your References section:

    Cell cycle-dependent expression of Dub3, Nanog and the p160 family of nuclear receptor coactivators (NCoAs) in mouse embryonic stem cells. van der Laan S, Golfetto E, Vanacker JM, Maiorano D. PLoS One. 2014 Apr 2;9(4):e93663. doi: 10.1371/journal.pone.0093663. eCollection 2014. PONE-D-14-02572 [pii] PubMed 24695638