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pLCKO
(Plasmid #73311)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 73311 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pLKO.1
  • Backbone manufacturer
    Broad Institute, Cambridge, MA
  • Backbone size (bp) 9436
  • Modifications to backbone
    The invariant gRNA scaffold was amplified from pX330-U6-Chimeric_BB-CBh-hSpCas9 (Addgene 42230) with 5’ BspAI/BfuAI sites and cloned into the pLKO.TRC005 lentiviral vector (Broad Institute, Cambridge, MA) between the AgeI and EcoRI sites. BspAI/ BfuAI sites were used to clone individual 20 bp gRNA sequences.
  • Vector type
    Mammalian Expression, Lentiviral, CRISPR
  • Promoter U6
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    NEB Stable
  • Copy number
    High Copy

Cloning Information

Resource Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pLCKO was a gift from Jason Moffat (Addgene plasmid # 73311 ; http://n2t.net/addgene:73311 ; RRID:Addgene_73311)
  • For your References section:

    High-Resolution CRISPR Screens Reveal Fitness Genes and Genotype-Specific Cancer Liabilities. Hart T, Chandrashekhar M, Aregger M, Steinhart Z, Brown KR, MacLeod G, Mis M, Zimmermann M, Fradet-Turcotte A, Sun S, Mero P, Dirks P, Sidhu S, Roth FP, Rissland OS, Durocher D, Angers S, Moffat J. Cell. 2015 Dec 3;163(6):1515-26. doi: 10.1016/j.cell.2015.11.015. Epub 2015 Nov 25. 10.1016/j.cell.2015.11.015 PubMed 26627737