PurposeExpression of STAT3-S727D
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||73364||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression
Growth in Bacteria
Gene/Insert nameSignal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)
SpeciesH. sapiens (human)
MutationE16K S727D E760D
Entrez GeneSTAT3 (a.k.a. ADMIO, ADMIO1, APRF, HIES)
- Promoter CMV
/ Fusion Protein
- FLAG tagged (C terminal on backbone)
- Cloning method Unknown
- 5′ sequencing primer CMV-F
- 3′ sequencing primer BGH-rev (Common Sequencing Primers)
E16K and E760D are present in other STAT3 isoforms and are believed to be SNPs.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pCDNA3-STAT3-S727D was a gift from Jie Chen (Addgene plasmid # 73364 ; http://n2t.net/addgene:73364 ; RRID:Addgene_73364)
For your References section:Signal transducer and activator of transcription 3 (STAT3) mediates amino acid inhibition of insulin signaling through serine 727 phosphorylation. Kim JH, Yoon MS, Chen J. J Biol Chem. 2009 Dec 18;284(51):35425-32. doi: 10.1074/jbc.M109.051516. Epub . 10.1074/jbc.M109.051516 PubMed 19875458