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pCDNA3-STAT3-S727D
(Plasmid #73364)

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Full plasmid sequence is not available for this item.

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 73364 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Signal Transducer And Activator Of Transcription 3 (Acute-Phase Response Factor)
  • Species
    H. sapiens (human)
  • Mutation
    E16K S727D E760D
  • Entrez Gene
    STAT3 (a.k.a. ADMIO, ADMIO1, APRF, HIES)
  • Promoter CMV
  • Tag / Fusion Protein
    • FLAG tagged (C terminal on backbone)

Cloning Information

Resource Information

Depositor Comments

E16K and E760D are present in other STAT3 isoforms and are believed to be SNPs.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pCDNA3-STAT3-S727D was a gift from Jie Chen (Addgene plasmid # 73364 ; http://n2t.net/addgene:73364 ; RRID:Addgene_73364)
  • For your References section:

    Signal transducer and activator of transcription 3 (STAT3) mediates amino acid inhibition of insulin signaling through serine 727 phosphorylation. Kim JH, Yoon MS, Chen J. J Biol Chem. 2009 Dec 18;284(51):35425-32. doi: 10.1074/jbc.M109.051516. Epub . 10.1074/jbc.M109.051516 PubMed 19875458