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Orai1-GCaMP6f
(Plasmid #73564)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 73564 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEGFP-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 3958
  • Total vector size (bp) 6166
  • Modifications to backbone
    The EGFP gene has been replaced with the genetically encoded calcium indicator GCaMP6f
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Orai1-GCaMP6f
  • Species
    H. sapiens (human), Synthetic
  • Insert Size (bp)
    2208
  • GenBank ID
    NM_032790.3
  • Entrez Gene
    ORAI1 (a.k.a. CRACM1, IMD9, ORAT1, TAM2, TMEM142A)
  • Promoter CMV Immediate Early
  • Tag / Fusion Protein
    • GCaMP6f (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer TGACGTCAATGGGAGTTTG
  • 3′ sequencing primer ACATTGATGAGTTTGGAC
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses

Depositor Comments

The fusion protein in this plasmid contains the genetically encoded calcium indicator GCaMP6f from the plasmid pGP-CMV-GCaMP6f (Addgene plasmid # 40755) provided by Douglas Kim. Note that the hOrai1 gene isolated in the Cahalan lab contains a silent mutation/polymorphism (T798C; aa T266).

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    Orai1-GCaMP6f was a gift from Michael Cahalan (Addgene plasmid # 73564 ; http://n2t.net/addgene:73564 ; RRID:Addgene_73564)
  • For your References section:

    Genetically targeted single-channel optical recording reveals multiple Orai1 gating states and oscillations in calcium influx. Dynes JL, Amcheslavsky A, Cahalan MD. Proc Natl Acad Sci U S A. 2016 Jan 12;113(2):440-5. doi: 10.1073/pnas.1523410113. Epub 2015 Dec 28. 10.1073/pnas.1523410113 PubMed 26712003